| Vector Information |
Size (kb): 6.8590002059936520 DESCRIPTION OF VECTOR:
Intact vector size: 6.859
Type of vector: phagemid
Cloning sites: HindIII BspMI PstI XbaI BamHI EcoRI
Polylinker sites: HindIII BspMI PstI SalI AccI XbaI BamHI AvaI XmaI SmaI EcoRI
ApaI
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...ApaI, ->, 1-63
promoter for in vitro transcription: SP6, <-, 68-84
terminator: SV40 polyadenylation
enhancer: SV40, 1099-1242
replicon: SV40, 1266-1401
marker(s): mycophenolic acid resistance (GPT), ->, 1614-2072
replicon: pMB1, <-, 3636-3636
marker(s): ampR, <-, 4394-5254
replicon: f1 ori, ->, 5385-5841
promoter for expression: CMV, ->, 6203-6790
promoter for in vitro transcription: T7, ->, 6834-6853 Vector: pWE1 (phagemid) Promoters: Promoter for in vitro transcription SP6 Marker(s):ampR,GPT Construct size (kb): 6.859000205993652 Features: marker(s): ampR
marker(s): mycophenolic acid resistance (GPT)
promoter for expression: CMV
promoter for in vitro transcription: SP6
promoter for in vitro transcription: T7
replicon: SV40
replicon: f1 ori
replicon: pMB1
MCS: HindIII...ApaI
terminator: SV40 polyadenylation
enhancer: SV40 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.8; SalI--3.7, 3.3. Having identical cloning sites also facilitates shuttling cDNAs from one vector to another. One of a series of vectors (ATCC 87671 - 87674) that allows simultaneous, high-level expression of multiple cDNA sequences in mammalian cells. A kit of all four vectors is also available (ATCC 87678). Each cDNA inserted into one of this series of vectors is regulated by an identical transcription unit, but each vector permits different selection to be applied to different subunits. |
