| 產(chǎn)品名稱 | Enterovirus D68 |
|---|---|
| 商品貨號 | B203957 |
| Classification | Picornaviridae, Enterovirus, Enterovirus D |
| Deposited As | Enterovirus 68 |
| Agent | Enterovirus D68 |
| Strain | Fermon |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen 1.0 mL per vial |
| Storage Conditions | -70°C or colder |
| Genome Sequenced Strain | Human enterovirus 68 strain Fermon, complete genome |
| Images |  |
| Comments | ATCC® VR-561™ was depositor material produced in primary monkey kidney cells. ATCC® VR-1826™ was created by serially passaging ATCC® VR-561™ four times onto MRC-5 (ATCC® CCL-171™) cells. |
| Effect on Host | CPE in cell culture |
| Recommended Host | MRC-5 (ATCC® CCL-171™) |
| Growth Conditions | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 2-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor | EH Lennette |
| Source | Isolated from a nasal-pharyngeal swab of a hospitalized 10-month-old female with pneumonia, California, 1962 |
| References | Schieble JH, et al. A probable new human picornavirus associated with respiratory diseases. Am. J. Epidemiol. 85: 297-310, 1967. PubMed: 4960233 |
| Cross References | Nucleotide (GenBank) : AY426531.1 Human enterovirus 68 strain Fermon, complete genome |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
