Restriction digests of the clone give the following sizes (kb): BamHI--5.6; HindIII--5.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid vector for genomic library construction. Insert-end-specific probes may be generated from T7 and SP6 promoters. Transcription terminators somewhat insulate the vector genes from insert transcription. |
