Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Protocol: ATCCNO: 50519 SPEC: The strain is routinely distributed as a frozen stabilate. See the general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing 10 ml of ATCC medium 1525. The baterial flora that is present in the shipped culture will support growth. Growth may be improved by using bacterized ATCC medium 1525 with a single species of bacteria. Bacterization is accomplished by inoculating the medium with Enterobacter aerogenes ATCC 13048 approximately 24 hours before use. Other species of bacteria may work equally well but this must be empirically assessed. The culture is routinely passaged every 14 days. This strain does not form a cyst stage and can be lost if it is not transferred regularly. To subculture, agitate and aseptically transfer 0.25 ml to 10 ml of freshly bacterized medium in a T-25 flask. Screw the cap on tightly and incubate at 25C.
Subcultivation
Protocol: ATCCNO: 50519 SPEC: The strain is routinely distributed as a frozen stabilate. See the general instructions for thawing and storage of frozen material before proceeding. Thaw the ampule and aseptically transfer the material to a T-25 tissue culture flask containing 10 ml of ATCC medium 1525. The baterial flora that is present in the shipped culture will support growth. Growth may be improved by using bacterized ATCC medium 1525 with a single species of bacteria. Bacterization is accomplished by inoculating the medium with Enterobacter aerogenes ATCC 13048 approximately 24 hours before use. Other species of bacteria may work equally well but this must be empirically assessed. The culture is routinely passaged every 14 days. This strain does not form a cyst stage and can be lost if it is not transferred regularly. To subculture, agitate and aseptically transfer 0.25 ml to 10 ml of freshly bacterized medium in a T-25 flask. Screw the cap on tightly and incubate at 25C.
Name of Depositor
S Tong
Year of Origin
1992
References
Tong S. Heterotrophic flagellates and other protists from Southampton water, U.K.. Ophelia 47: 71-131, 1997.
