| Insert |
DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: DNA segment, Chr 5, human D4S62 [D4S62] Alleles: b, s, A1, A2, A3, A4, B1, B2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/EcoRI--4.2, 2.2; BamHI--6.6; BglI--2.4, 2.1, 1.0, 0.4, 0.3; PstI/XbaI--5.1, 1.5; HindIII--uncut. The insert contains internal BglI, BglII, XbaI and SacII sites. The genomic copy number in monkey is less than 5. Detects African green monkey genomic fragments of the following sizes (approximate kb): BamHI--4.6; HindIII--19; KpnI--22; KpnI/HindIII--19; EcoRI--2.5; EcoRI/KpnI--2.5; BglII--2.2. Detects genomic EcoRI fragments of the following sizes (kb): human--2.6; mouse--4.4, 3.1, 3.0, 2.3; Chinese hamster--9.0, 6.6. Subcloned from lambdaMk8, a clone identified as being adjacent to monkey deca-satellite sequences. Enzyme(s) not detecting polymorphism: AluI, ApaI, AvaI, BamHI, BclI, BglII, EcoRI, HaeIII, HindIII, HinfI, KpnI, MboI, NdeI, PvuII, PstI, SstI, TaqI, XhoI, XhoII, XmnI. |
| References |
Maresca A, et al. Genomic organization of the low copy number sequences that are associated with deca-satellite DNA in the monkey genome. Nucleic Acids Res. 15: 8799-8813, 1987. PubMed: 2825119
Thayer RE, et al. Localization of a unique DNA sequence to band p16 of human chromosome 4. Cytogenet. Cell Genet. 45: 75-79, 1987. PubMed: 3476254
Hayden MR, et al. A polymorphic DNA probe located to human chromosome 4p16. Nucleic Acids Res. 15: 3938, 1987. PubMed: 2884639
Ronald E Thayer, personal communication
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