Restriction digests of the clone give the following sizes (kb): PvuI--1.9, 0.9; EcoRI--2.8; HindIII--2.8; NotI--2.8; SspI--2.8. The plasmid contains the following restriction sites (bp from 0): DrdI--91, 992; KasI--233; PvuI--276, 2150; EcoRI--396; NotI--418; NsiI--493; HindIII--531; SapI--767; BsaI--1850; SspI--2585. Cloning vector with the multiple cloning site expanded by inserting an oligonucleotide at the SmaI site of pUC19, thereby generating an amber mutation in the lacZ' coding sequence. The order of the major features in this plasmid is: KasI - 3' lacZ'amb1 - EcoRI/MCS/HindIII - 5' lacZ'amb1 - SapI - pMB1 ori - ampR. |
