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熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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Keratinocyte Growth Kit
Keratinocyte Growth Kit
規格:
貨期:
編號:B205634
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


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      產品名稱
      Keratinocyte Growth Kit
    
      
    
      
      商品貨號
      B205634
    
      
      
      
      Biosafety Level
      1 


      

      Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
      		
    
      
      
      
      Product Format
      frozen 1 Kit
    
      
      
      
      Storage Conditions
      -20°C (or -70°C for long-term storage)
    
      
      
      
      Comments
      
      The Keratinocyte Growth Kit contains components that when added to Dermal Cell Basal Medium (ATCC PCS-200-030) create a complete serum-free culture environment for keratinocytes. The complete keratinocyte growth medium is formulated to inhibit fibroblast growth, and the low calcium concentration (0.06 mM) slows differentiation. No feeder layers, extracellular matrix proteins or other substrates are required. The final concentration for each component in complete keratinocyte growth medium is as follows:
      

        
    
      • Extract P: 0.4%
        • 
    
      • rh TGF-alpha: 0.5 ng/mL
        • 
    
      • L-Glutamine: 6 mM
        • 
    
      • Hydrocortisone: 100 ng/mL
        • 
    
      • Insulin: 5 µg/mL
        • 
    
      • Epinephrine: 1.0 µM
        • 
    
      • Apo-transferrin: 5 µg/mL
        • 

      		
    
      
      
      
      Complete Growth Medium
      
        
    
      1. 
    
        Obtain one growth kit from the freezer; make sure that the caps of all components are tight. 
        
    
        2. 
    
      2. 
    
        Thaw the components of the growth kit just prior to adding to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
        
    
        3. 
    
      3. 
    
        Obtain one bottle of Dermal Cell Basal Medium (485 mL) from cold storage. 
        
    
        4. 
    
      4. 
    
        Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying with 70% ethanol. 
        
    
        5. 
    
      5. 
    
        Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component to the bottle of basal medium using a separate sterile pipette for each. 
        
    
        6. 
    
        Table 1. Keratinocyte Growth Kit Components  
        
    
        
        
            
        
                
                
        Component
        		
    
        
      
        
      Volume
      
        Volume
        		
    
        
      
        
      Sterility Tests
      
        Final Concentration
        		
    
        
      
        
      Functional Tests
      
        Extract P
        		
    
        
      
        
      C of A
      
        2.0 mL
        		
    
        
            

        

						
					
					
        
							
        
							  
							  
							    
        
							      梅經理
							      17280875617
							      1438578920
							    
        
							    
        
							      胡經理
							      13345964880
							      2438244627
							    
        
							    
        
							      周經理
							      17757487661
							      1296385441
							    
        
							    
        
							      于經理
							      18067160830
							      2088210172
							    
        
							    
        
							      沈經理
							      19548299266
							      2662369050
							    
        
							    
        
							      李經理
							      13626845108
							      972239479
							    
        
							    
							          
							
        
					
        
				
				
        
					
				
        
			
			
		
		
        
	
  
        
	






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