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pWL102 [ WFD1005]
pWL102 [ WFD1005]
規(guī)格:
貨期:
編號(hào):B205762
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pWL102 [ WFD1005]
商品貨號(hào) B205762
Designations pWL102 [DSM5715, WFD1005]
Depositors WF Doolittle
Biosafety Level 1
Vector Information
Size (kb): 10.5000000000000000
Vector: pWL102 (plasmid)
Construction: pHV2, pAT153, Haloferax volcanii DS2
Marker(s):ampR,mevR
Construct size (kb): 10.5
Features: marker(s): ampR, mevR
replicon: pHV2, pMB1
Applications
contains sequence 3-hydroxy-3-methylglutaryl-Coenzyme A reductase
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--11.0; HindIII--6.6, 4.1; KpnI/SphI--7.2, 3.6; PstI--7.6, 3.0; XbaI--11.0.
The 3.5 kb SphI/KpnI fragment conferring mevinolin resistance has been sequenced. The mutation in the hmgA gene is at position -29 relative to the transcription start site, and results in an increased level of transcription.
Shuttle vector with demonstrated ability to transform Haloferax volcanii, Haloferax mediterranei (ATCC 33500), Halobacterium halobium, Haloarcula hispanica (ATCC 33960), and Haloarcula vallismortis (ATCC 29715).
The order of the major features in this plasmid is: NcoI - BamHI - HindIII - XbaI - KpnI - mevR - sphI - pMB1 ori - ampR - EcoRI - ClaI - HindIII - pHV2 fragment.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Brooun A, et al. Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinrrium. J. Bacteriol. 179: 2963-2968, 1997. PubMed: 9139915

Cline SW, Doolittle WF. Transformation of members of the genus Haloarcula with shuttle vectors based on Halobacterium halobium and Haloferax volcanii plasmid replicons. J. Bacteriol. 174: 1076-1080, 1992. PubMed: 1732202

Lam WL, Doolittle WF. Mevinolin-resistant mutations identify a promoter and the gene for a eukaryote-like 3-hydroxy-3-methylglutaryl-coenzyme A reductase in the archaebacterium Haloferax volcanii. J. Biol. Chem. 267: 5829-5834, 1992. PubMed: 1556098

Lam WL, Doolittle WF. Shuttle vectors for the archaebacterium Halobacterium volcanii. Proc. Natl. Acad. Sci. USA 86: 5478-5482, 1989. PubMed: 2748598

Shipped frozen
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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