Restriction digests of the clone give the following sizes (kb): ClaI--8.0, 4.1; BamHI--uncut; HindIII--3.9, 2.3, 2.1, 1.9, 1.0, 0.9. Can be mobilized into F. diplosiphon using the conjugative plasmid RP4. Shuttle vector. A 2.9 kb BamHI/ClaI fragment from a different construct containing the origin of replication has been sequenced. The BamHI site is not retained in this plasmid. Constructed by inserting a 8.0 kb ClaI fragment, containing the F. diplosiphon pFDA origin of replication, into the ClaI site of pJCF22. The order of the major features of the plasmid is: cmlR - bom - oriV - F. diplosiphon pFDA ori. |
