| Vector |
Construct size (kb): 17.29998970031738 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pTZ18R
Intact vector size: 2.900
Type of vector: phagemid
Vector end: NotI
Vector end: NotI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI SalI AccI HincII PstI SphI
HindIII
Construction: pUC18, Escherichia coli lacZalpha, pBR322, f1
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: LDLR
Genomic copy number: unique
Gene name: low density lipoprotein receptor (familial
hypercholesterolemia)
Contains complete coding sequence?: U
Chromosome: 19; Localization: 19 p13.2
Type of DNA: genomic
Insert end: NotI
Insert end: NotI
Insert size (kb): 14.4
Cross references: Insert lengths(kb): 14.39999961853027 Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) [LDLR] Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1 Target Gene: low density lipoprotein receptor (familial hypercholesterolemia) |
| Comments |
Restriction digests of the clone give the following sizes (kb): SalI--14.8, 2.5; EcoRI--12.8, 4.5; BglII--14.0, 3.3; SstI--17.3; NotI--14.4; 2.9. The insert includes the following restriction sites: SalI (intron 1, 2.0 kb from the 5' NotI site); BglII (intron 3); EcoRI (cDNA segment). Encodes the normal sequence. The expected size of the correctly processed mRNA is 2.9 kb. The NotI fragment consists of the following elements: the mouse metallothionein promoter I (1.7 kb); exon 1 of LDLR, containing a SstII site; intron 1 truncated by deletion of a RsaI (77 nt 3' to exon 1) / EcoRI (3.1 kb 5' to exon 2) fragment; exons and introns 2-4; a cDNA fragment corresponding to exons 5-18; and transcription termination and polyadenylation signals from the human growth hormone receptor gene (0.6 kb). |
| References |
Pathak RK, et al. Tissue-specific sorting of the human LDL receptor in polarized epithelia of transgenic mice. J. Cell Biol. 111: 347-359, 1990. PubMed: 2199454
Masayuki Yokode, personal communication
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