Restriction digests of the clone give the following sizes (kb): EcoRI--4.7; HindIII--4.7; EcoRI/SmaI--4.7. Should be grown in media containing ampicillin at a final concentration of 1 mg/ml to improve plasmid yields. May be used in essentially all cultured cells in transient transfection assays, to establish permanent cell lines and to construct transgenic mice. The pCMV1, 2, 3, 5 and 6 vectors differ from each other in certain unique restriction sites in the polylinker region. |
