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pCu414CTR1-CYC1
pCu414CTR1-CYC1
規格:
貨期:
編號:B207177
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pCu414CTR1-CYC1
商品貨號 B207177
Designations pCu414CTR1-CYC1
Depositors S Labbe, DJ Thiele
Biosafety Level 1
Vector Information
Size (kb): 5.3949999809265140
Vector: pCu414CTR1-CYC1 (phagemid)
Promoters: Promoter for expression CTR1-CYC1
Construction: pRS414, CTR1-CYC1 promoter
Marker(s):TRP1,ampR
Construct size (kb): 5.394999980926514
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CTR1-CYC1
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: XhoI...SpeI
terminator: CYC1
centromere: CEN6
Applications
cloning vector
Comments
Restriction digests of the vector give the following sizes (kb): SacI--5.4; SacI/SpeI--5.0, 0.38.
CTR3 and CTR1 are the copper transporter promoters and are repressed by the presence of copper and highly activated under copper-starvation conditions. The time course of copper-starvation activation and copper repression of a reporter gene is rapid.
References

Labbe S, Thiele DJ. Copper ion inducible and repressible promoter systems in yeast. Methods Enzymol. 306: 145-153, 1999. PubMed: 10432452

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