| Vector |
Construct size (kb): 7.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: SalI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: SREBF1
Other gene symbols: SREBP1A
Gene name: sterol regulatory element binding transcription factor 1
Contains complete coding sequence?: Y
Chromosome: 17
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 4.2
Cross references: DNA Seq. Acc.: U00968 Insert lengths(kb): 4.199999809265137 Gene product: sterol regulatory element binding transcription factor 1( SREBF1 sterol regulatory element binding transcription factor 1, SREBP1, sterol regulatory element binding protein-1) [SREBF1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/SalI--4.2, 3.2; EcoRI--4.3, 2.9; KpnI--6.0, 1.1, 0.4; EcoRI/SalI--2.8, 2.7, 1.5; SacI--6.0, 0.7, 0.5. Restriction digests of the clone give the following sizes (kb): EcoRI--4.4, 2.7; KpnI--5.6, 1.2, 0.34; BamHI/SalI--4.2, 2.9; EcoRI/SalI--2.9, 2.7, 1.5; EcoRI--Eco47III--4.4, 1.5, 1.2. The insert contains the following restriction sites (kb from the 5' end): Eco47III--1.174; EcoRI--2.668; KpnI--2.685, 3.021. Constructed with an EcoRI linker at the 5' end and an EcoRI(NotI) adaptor at the 3' end. |
| References |
Yokoyama C, et al. SREBP-1, a basic helix-loop-helix leucine zipper protein that controls transcription of the low density lipoprotein receptor gene. Cell 75: 187-197, 1993. PubMed: 8402897
Joseph L Goldstein, personal communication
|
