| 產品名稱 |
pEW403 [LEW403] |
| 商品貨號 |
B207588 |
| Designations |
pEW403 [LEW403] |
| Depositors |
DF Barker |
| Vector |
Construct size (kb): 7.30
Name of vector: pUC8
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Construction: pBR322, Escherichia coli lacZalpha, M13mp8
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none |
| Insert |
Genome: Homo sapiens
Gene symbol: D17S63
Genomic copy number: unique
Gene name: DNA Segment, single copy
Contains complete coding sequence?: U
Chromosome: 17
Localization: 17 p12-p11.2
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 4.6
Cross references:
Restriction enzyme: MspI
Alleles: A2, A1 |
| Insert Size (kb) |
4.60 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Plasmid in Escherichia coli JM83 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.9, 2.7; HindIII--5.2, 2.2; BamHI--3.0, 2.5, 2.2; SmaI--7.7; SalI--7.0. This probe contains repetitive sequences. This probe may hybridize to a non 17-specific human fragment, but the polymorphism is linked to 17-specific markers. |
| References |
Wright EC, et al. A genetic map of human chromosome 17p. Genomics 7: 103-109, 1990. PubMed: 2335351
David F Barker, personal communication
|
