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pBSE36
pBSE36
規格:
貨期:
編號:B208184
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pBSE36
商品貨號 B208184
Designations pBSE36
GenBank Number

M34077

Species tobacco mild green mosaic tobamovirus (TMGMV)
Depositors F Garcia-Arenal
Vector
Construct size (kb): 5.099999904632568
Insert
DNA: cDNA
Insert lengths(kb): 1.871999979019165
Gene product: genome, partial
Insert Size (kb) 1.872
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.2, 2.0; PstI--3.4, 1.7; PvuII--2.8, 2.3.
Insert corresponds to nt 1990 to nt 3862 of the genome. This includes coding sequences for the 183 kDa protein (exons nt 71 to nt 3403 and nt 3407 to nt 4900) and the 126 kDa protein (nt 71 to nt 3406).
Linearizing with HindIII and transcription from the T7 promoter gives the minus (antisense) strand. Linearizing with EcoRI and transcription from the T3 promoter gives the plus (sense) strand.
Classification Tobamovirus
References

Garcia-Arenal F. Sequence and structure at the genome 3' end of the U2-strain of tobacco mosaic virus, a histidine-accepting tobamovirus. Virology 167: 201-206, 1988. PubMed: 3188396

Dracopoli NC, et al. The complete nucleotide sequence of the genomic RNA of the tobamovirus tobacco mild green mosaic virus. Virology 177: 553-558, 1990. PubMed: 2371769

F Garcia-Arenal, personal communication

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