Restriction digests of the clone give the following sizes (kb): XbaI--8.4; PstI--8.4; EcoRI--8.4; HindIII--8.4. There are restriction sites for KpnI (2 sites), SpeI (2 sites), and EcoRV (1 site) in the Lys2 gene that are also present in the polylinker. One of a series of pBluescript-based centromere vectors (ATCC 77142-77145, 77157-77158) differing in the yeast selectable marker gene. YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. Useful in plasmid shuffle experiments. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). The order of the major features in this plasmid is: LYS2 - f1 ori (NaeI) - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori bla - CEN6 - ARSH4. |
