| Insert |
DNA: genomic Insert lengths(kb): 33.59999847412109 Gene product: DNA Segment, single copy [D16S60] Alleles: A1, A2, A3, B1, B2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--9.0, 7.2, 4.3 (doublet), 4.1, 3.5, 2.3, 2.0; BglII--9.3 (doublet), 6.0, 5.6, 3.7, 3.2, 2.0, 1.9; EcoRI--6.2, 5.6, 4.8, 3.5, 2.9 (doublet), 2.6 (doublet), 2.1, 2.0 (doublet), 1.7, 0.9; HindIII--16.0, 13.0, 9.0, 7.0; BamHI--23.3, 13.0, 2.2. The insert can be excised from the vector with EcoRI, resulting in a 5.5 kb EcoRI vector fragment. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The HincII polymorphisms have a combined PIC value of 0.81 and a combined heterozygosity of 83%. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, PstI, RsaI (panel of 5 individuals). |
| References |
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
Keith TP, et al. Genetic linkage map of 43 DNA markers on human chromosome 16. Proc. Natl. Acad. Sci. USA 87: 5754-5758, 1990. PubMed: 2377614
Reeders ST, et al. Regional localisation of the autosomal dominant polycystic kidney disease locus. Genomics 3: 150-155, 1988. PubMed: 2906325
Jacobsen SJ, et al. A clinically homogeneous group of families with facioscapulohumeral (Landouzy-Dejerine) muscular dystrophy: linkage analysis of six autosomes. Am. J. Hum. Genet. 47: 376-388, 1990. PubMed: 1975474
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