Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
dry soil, Mexico City, Mexico
Product Format
frozen
Type Strain
yes
Comments
Originally deposited as Pinaciophora sp. Name changed to Nerada mexicana as described in Cavalier-Smith and Chao (J Mol Evol 2006 62:388-420).
Medium
ATCC® Medium 1967: Soil freshwater cereal grass medium
Growth Conditions
Temperature: 25.0°C
Protocol: This strain is shipped as a frozen stabilate on dry ice. Please read the instructions that accompany the shipment before handling the material. Thaw the vial as instructed and transfer its entire contents to 10 ml of ATCC medium 1967 in a T-25 tissue culture flask. The bacterial flora present in the shipped culture will support growth, but growth may be improved by using medium that has been bacterized with Enterobacter aerogenes ATCC 13048 approximately 24 hours before inoculation with the protist. (Other species of bacteria may work equally well, but this one has been empirically assessed.) Subculture every 14-21 days. To subculture, aseptically scrape the bottom of the flask, agitate the flask to distribute the cells evenly, and aseptically transfer 0.25 ml to 10 ml of bacterized medium in a T-25 flask. Screw the cap on tightly and incubate the flask at 25C.
Subcultivation
Protocol: This strain is shipped as a frozen stabilate on dry ice. Please read the instructions that accompany the shipment before handling the material. Thaw the vial as instructed and transfer its entire contents to 10 ml of ATCC medium 1967 in a T-25 tissue culture flask. The bacterial flora present in the shipped culture will support growth, but growth may be improved by using medium that has been bacterized with Enterobacter aerogenes ATCC 13048 approximately 24 hours before inoculation with the protist. (Other species of bacteria may work equally well, but this one has been empirically assessed.) Subculture every 14-21 days. To subculture, aseptically scrape the bottom of the flask, agitate the flask to distribute the cells evenly, and aseptically transfer 0.25 ml to 10 ml of bacterized medium in a T-25 flask. Screw the cap on tightly and incubate the flask at 25C.
Name of Depositor
J Martinez-Cruz
Chain of Custody
ATCC <<--J Martinez-Cruz<<--T.A. Nerad
References
Jovita Martinez-Cruz, personal communication
Cavalier-Smith, T and EE Chao. Phylogeny and megasystematics of phagotrophic heterokonts (kingdom Chromista). J Mol Evol 62:388-420, 2006. PubMed: 16557340
