| Insert |
DNA: genomic Insert lengths(kb): 1.799999952316284 Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) [RRM1] Alleles: A2, A2, A1 |
| Comments |
The insert contains the following restriction sites (in nt from the EcoRI site, closer to the XhoI site of the vector): BglII--50; NdeI--201, 907. AatII, ApaI, BamHI, EcoRV, EcoRI, HindIII, Not I, SacI, XbaI and XhoI do not cut the insert. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.8; BamHI--4.8; SacI--4.8; XhoI--4.8; PstI--uncut. The 1.8 kb EcoRI fragment (pE1.8, ATCC 65264/65265) is immediately adjacent to and downstream from a 2.8 kb EcoRI fragment (pE2.8, ATCC 65260/65261). Detects the same 15.5 kb SacI band as pE2.8. |
| References |
Byrne J, Smith P. Human polymorphic probe pE1.8 detects SacI polymorphism in the ribonucleotide reductase M1 subunit gene. Hum. Genet. 87: 376, 1991. PubMed: 1677928
Jennifer A Byrne, personal communication
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