Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.7, 1.0, 0.4; EcoRV--5.0, 1.4; HindIII--5.2, 1.2. Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
