| 產品名稱 |
pSE1112 |
| 商品貨號 |
B211691 |
| Designations |
pSE1112 |
| Species |
Homo sapiens, human |
| Depositors |
SJ Elledge |
| Applications |
in another host, produces protein SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAS1
Intact vector size: 7.100
Type of vector: phagemid
Vector end: BamHI
Vector end: BamHI
Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI
Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 DNA-binding domain, ->
epitope tag: hemagglutinin (HA), ->
restriction site: EcoRI, ->, 4315
MCS: NdeI...SalI, ->, 4395-4422
terminator: ADC1
replicon: pMB1
marker(s): ampR
replicon: 2 micron
marker(s): TRP1, ->
replicon: f1
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: SNF1
Gene name: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.3
Cross references: Insert lengths(kb): 2.299999952316284 Gene product: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase [SNF1] Target Gene: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Insert Size (kb) |
2.300 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; HindIII--5.8, 3.4; BamHI--7.0, 2.3. pSE1111 (ATCC 87010) and pSE1112 (ATCC 87011) can be used together as a positive control in 2-hybrid cloning systems. Expresses SNF1 fused to the GAL4 DNA-binding domain. |
| References |
Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581
Stephen J Elledge, personal communication
|
