| 產品名稱 |
pNAK28 |
| 商品貨號 |
B211754 |
| Designations |
pNAK28 |
| GenBank Number |
V01555
|
| Species |
Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus |
| Depositors |
Johns Hopkins University, G Milman, Johns Hopkins University |
| Applications |
produces protein nuclear antigen 1 |
| Vector |
Construct size (kb): 6.199999809265137 |
| Insert |
DNA: genomic Insert lengths(kb): 2.236000061035156 Tissue: P3HR-1 cell line Gene product: nuclear antigen 1 [EBNA-1] Target Gene: nuclear antigen 1 |
| Insert Size (kb) |
2.236 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.7, 2.4; BglI--4.9, 1.2; SmaI--3.7, 2.4. pHE6 is a high-expression plasmid derived from pUC8. Inserts are expressed as run-on products initiated at lambda N. Synthesis is regulated by the cI857 repressor encoded by the plasmid. Corresponds to nt 109241-111960 of the GenBank record and includes a portion of the EBNA coding region (109298-109872, the carboxy-terminal third. Expresses a 28 kDa fusion protein composed of 36 amino acids from lambda N protein and linker and 191 amino acids of EBNA sequence. The protein is recognized by anti-EBNA antibodies and includes the DNA-binding domain that interacts with oriP. This SmaI fragment is downstream of the triplet repeat region in the BamHI K fragment. |
| Classification |
Herpesviridae, Lymphocryptovirus |
| References |
Milman G. Expression of antigenic epstein-barr virus polypeptides in bacteria and their use in diagnostics. US Patent 5,256,768 dated Oct 26 1993
Milman G, et al. Carboxyl-terminal domain of the Epstein-Barr virus nuclear antigen is highly immunogenic in man. Proc. Natl. Acad. Sci. USA 82: 6300-6304, 1985. PubMed: 2412231
Baer R, et al. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310: 207-211, 1984. PubMed: 6087149
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
