| Vector |
Construct size (kb): 12.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAcMP3
Intact vector size:
Type of vector: plasmid
Vector end: XbaI
Vector end: XbaI
Cloning sites:
Polylinker sites: BamHI XbaI EcoRI NotI EagI PstI BglII
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
promoter: basic protein, ->
marker(s): ampR
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCH
Genomic copy number: unique
Gene name: protein kinase C, eta
Contains complete coding sequence?: Y
Tissue: keratinocytes
Type of DNA: cDNA
Insert end: NheI
Insert end: NheI
Insert size (kb): 2.0
Cross references: Insert lengths(kb): 2.0 Tissue: keratinocytes Gene product: protein kinase C, eta [PRKCH] Target Gene: protein kinase C, eta |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 1.3, 0.55; BglII--11.0, 0.9; PvuI--6.0, 4.0, 1.5, 0.95; PvuII--3.2, 3.0, 2.4, 1.8, 1.4, 0.45; SacI--12.0. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.21, 1.75; BglII--1.43; PvuI--1.28; PvuII--0.12, 1.86; SacI--1.37. Constructed using NheI adaptors. Most of the insert (all but approximately 250 bp) can be excised using BamHI, but the insert will be in two fragments- 1.2 and 0.55 kb. Contains the complete coding sequence only. |
| References |
Rice JW, et al. A comparison of large-scale Sf9 insect cell growth and protein production: stirred vessel vs. airlift. BioTechniques 15: 1052-1059, 1993. PubMed: 8292338
David J Burns, personal communication
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