Tritrichomonas foetus (Riedmuller) Wenrich and Emerson
商品貨號
B211809
Strain Designations
KV-1
Application
produces glucokinase
produces ketohexokinase fructokinase
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ATCC® Medium 2154: LYI Entamoeba medium
ATCC® Medium 359: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 7.2 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use
Growth Conditions
Temperature: 35°C
Culture System: Axenic
Subcultivation
Protocol: at pH 7.0
Cryopreservation
Harvest and Preservation
Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. When cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.
Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.
Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube;
Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium;
Invert several times to dissolve the DMSO;
Allow to warm to room temperature.
Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.
Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 359 (completed with serum) or 13 mL ATCC Medium 2154.
Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154).
Name of Depositor
BM Honigberg, J Kulda
Chain of Custody
ATCC <-- BM Honigberg, J Kulda <-- E. Lipova
Year of Origin
1962
References
Beach DH, et al. Phospholipid metabolism of cultured Trichomonas vaginalis and Tritrichomonas foetus. Mol. Biochem. Parasitol. 44: 97-108, 1991. PubMed: 2011157
Beach DH, et al. Fatty acid and sterol metabolism of cultured Trichomonas vaginalis and Tritrichomonas foetus. Mol. Biochem. Parasitol. 38: 175-190, 1990. PubMed: 2325705
Kulda J, Honigberg BM. Behavior and pathogenicity of Tritrichomonas foetus in chick liver cell cultures. J. Protozool. 16: 479-495, 1969. PubMed: 5343462
Mertens E, Muller M. Glucokinase and fructokinase of Trichomonas vaginalis and Tritrichomonas foetus. J. Protozool. 37: 384-388, 1990. PubMed: 2213652
Gunderson J, et al. Phylogeny of trichomonads inferred from small-subunit rRNA sequences. J. Eukaryot. Microbiol. 42: 411-415, 1995. PubMed: 7620466
Delgado-Viscogliosi P, et al. Electrophoretic mobility of tubulin subunits as a criterion for testing relationships between trichomonad taxa. Arch. Protistenkd. 146: 191-200, 1995.
Ashton DH. Metronidazole resistant tritrichomonas foetus activities of hydrogenosomal enzymes in course of development of anaerobic resistance. Acta Univ. Carol. Biol. 30: 513-519, 1986.
Yarlett N, et al. Subcellular localization of the enzymes of the arginine dihydrolase pathway in Trichomonas vaginalis and Trtrichomonas foetus. J. Eukaryot. Microbiol. 41: 554-559, 1994. PubMed: 7866382
Mertens E, et al. Presence of a fructose-2,6-bisphosphate-insensitive pyrophosphate: fructose-6-phosphate phosphotransferase in the anaerobic protozoa Tritrichomonas foetus, Trichomonas vaginalis and Isotricha prostoma. Mol. Biochem. Parasitol. 37: 183-190, 1989. PubMed: 2558319
Viscogliosi E, et al. Phylogenetic implication of iron-containing superoxide dismutase genes from trichomonad species. Mol. Biochem. Parasitol. 80: 209-214, 1996. PubMed: 8892298
Felleisen RS. Comparative genetic analysis of tritrichomonadid protozoa by the random amplified polymorphic DNA technique. Parasitol. Res. 84: 153-156, 1998. PubMed: 9493217
Felleisen RS. Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa. Parasitology 115: 111-119, 1997. PubMed: 10190167
Vanacova S, et al. Characterization of Trichomonad species and strains by PCR fingerprinting. J. Eukaryot. Microbiol. 44: 545-552, 1997. PubMed: 9435127
Viscogliosi E, Mueller M. Phylogenetic relationships of the glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, from parabasalid flagellates. J. Mol. Evol. 47: 190-199, 1998. PubMed: 9694668
Brugerolle G, et al. Centrin protein and genes in Trichomonas vaginalis and close relatives. J. Eukaryot. Microbiol. 47: 129-138, 2000. PubMed: 10750840
Mattos A, et al. Fine structure and isozymic characterization of trichomonadid protozoa. Parasitol. Res. 83: 290-295, 1997. PubMed: 9089728
Bouma MJ, et al. Activity of disulfiram (bis(diethylthiocarbamoyl)disulphide) and ditiocarb (diethyldithiocarbamate) against metronidazole-sensitive and -resistant Trichomonas vaginalis and Tritrichomonas foetus. J. Antimicrob. Chemother. 42: 817-820, 1998. PubMed: 10052908
Tachezy J, et al. Cattle pathogen Tritrichomonas foetus (Riedmuller, 1928) and pig commensal Tritrichomonas suis (Gruby & Delafond, 1843) belong to the same species. J. Eukaryot. Microbiol. 49: 154-163, 2002. PubMed: 12046599
Gerbod D, et al. Phylogenetic relationships of class II fumarase genes from trichomonad species. Mol. Biol. Evol. 18: 1574-1584, 2001. PubMed: 11470849
Land KM, et al. Loss of multiple hydrogenosomal proteins associated with organelle metabolism and high-level drug resistance in trichomonads. Exp. Parasitol. 97: 102-110, 2001. PubMed: 11281707
Brugerolle G, et al. Immunolocalization of two hydrogenosomal enzymes of Trichomonas vaginalis. Parasitol. Res. 86: 30-35, 2000. PubMed: 10669133
Singh BN, et al. Immunological and biochemical analysis of glycosylated surface antigens and lipophosphoglycan of Tritrichomonas foetus. J. Parasitol. 87: 770-777, 2001. PubMed: 11534640
Land KM, et al. In vivo expression of ferredoxin in a drug resistant trichomonad increases metronidazole susceptibility. Mol. Biochem. Parasitol. 121: 153-157, 2002. PubMed: 11985873
Cross References
Nucleotide (GenBank) :
Z70668
T.foetus sod1 gene.
Nucleotide (GenBank) :
Z70669
T.foetus sod2 gene.
Nucleotide (GenBank) :
AJ249457
mRNA for centrin (ce1 gene)
