| 產品名稱 |
Verticillium dahliae Klebahn |
| 商品貨號 |
B212176 |
| Deposited As |
Verticillium dahliae Klebahn, anamorph |
| Strain Designations |
Brm-1 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
freeze-dried |
| Type Strain |
no |
| Preceptrol® |
no |
| Comments |
Melanin biosynthesis Accumulates (+)-scytalone and flaviolin Brown microsclerotia |
| Morphology |
Colonies on potato dextrose agar are grey to olivaceous, powdery or felty. Conidiophores mostly unbranched, with one or few conidial scars, up to 50 μm long, 3-6 μm wide. Conidial chains profusely branched. Conidia obclavate to ellipsoidal, with a short, cylindrical beak, 23-56 x 8-17 μm, medium brown, rugulose with muriform septation, with a single scar at the tip, arising in mostly unbranched chains of ten or more. |
| Medium |
ATCC® Medium 336: Potato dextrose agar (PDA)
|
| Growth Conditions |
Temperature: 24.0°C |
| Sequenced Data |
???? 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. TATTGATATGCTTAAGTTCAGCGGGTATTCCTACCTGATCCGAGGTCAACCGTTGCCGCACGAGGCGGGCTTGTAGGGGGTTTAGAGGCAAGCGCCTGCGGGACTCCGATGCGAGCTGTAACTACTACGCAAGGAAGGGCCACGCGGGTCCGCCACTGCTTTTAAGGGCCTACAGACGTAGATCCCCAACACCGGGCCACTGGGGCTCGAGGGTTGAAACGACGCTCGGACAGGCATGCCTCCCAGGATACTGGAAGGCGCCATGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGCAATTCACACTACATATCGCGTTTCGCTGCGTTCTTCATCGATGCTAGAGCCAAGAGATCCGTTGTTAAAAGTTTTAATAGTTCGCTAAGAACACTCAGAAGTATCGTTGGTATAAACAGAGAGACTGATGGACCGGCGGGCTCGCAGAACGAGCCGCCGAAGCAACAATATGGTTCACAAAGGGTTATGAGTAGATACTCGGTAATGATCCCTCCGCAG |
| Name of Depositor |
MH Wheeler |
| Chain of Custody |
ATCC <<--MH Wheeler<<--J. Puhalla |
| Isolation |
UV-induced mutant of ATCC 44574 |
| Cross References |
Nucleotide (GenBank) :
JQ070078
ITS including 5.8S rRNA gene
|
| References |
Geis PA, et al. Pentaketide metabolites of melanin synthesis in the dematiaceous fungus Wangiella dermatitidis. Arch. Microbiol. 137: 324-328, 1984. PubMed: 6539583
Wheeler MH, et al. Ultrastructure of melanin formation in Verticillium dahliae with (+)-scytalone as a biosynthetic intermediate. Can. J. Microbiol. 22: 702-711, 1976. PubMed: 945119
Bell AA, et al. Use of mutants to establish (+)-scytalone as an intermediate in melanin biosynthesis by Verticillium dahliae. Can. J. Microbiol. 22: 787-799, 1976. PubMed: 945120
Wheeler MH, et al. Ultrastructural and chemical distinction of melanins formed by Verticillium dahliae from (+)-scytalone, 1,8-dihydroxynaphthalene, catechol, and L-3,4-dihydroxyphenylalanine. Can. J. Microbiol. 24: 289-297, 1978. PubMed: 565670
|
