| 產品名稱 |
Herpesvirus saimiri |
| 商品貨號 |
B212631 |
| Classification |
Herpesviridae, Rhadinovirus |
| Agent |
Herpesvirus saimiri |
| Strain |
C-488 |
| Common Name |
Herpesvirus saimiri |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
This item contains FBS of Guatemalan origin. Strain is also available as ATCC® VR-1414™, which does not require a USDA permit due to multiple passages of ATCC® VR-1396™ in US origin FBS.
Herpesvirus saimiri strain C488 transforms human T lymphocytes to antigen- and mitogen-independent, but IL-2 dependent stable growth. Virus stocks are stable for months at 4C, but titer greatly reduced by freeze-thaw cycles.
For methods of propagation see: Fickenscher and Fleckenstein, 1994. in: Methods in Molecular Genetics. (ed) K. W. Adolph, 4: 345-362, 1994. |
| Effect on Host |
CPE, foci of detached cells bordered by rounded enlarged cells |
| Recommended Host |
OMK (ATCC® CRL-1556™)
|
| Growth Conditions |
Temperature: 37°C
Incubation: Incubate infected culture for 10-20 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance
|
| Name of Depositor |
M Daniel, R Desrosiers, B Biesinger, B Fleckenstein, H Fickenscher |
| Special Collection |
NCRR Contract |
| Source |
Blood lymphocytes of New England Regional Primate Research Ctr. squirrel monkey (Saimiri sciureus) |
| References |
Biesinger B, et al. The divergence between two oncogenic Herpesvirus saimiri strains in a genomic region related to the transforming phenotype. Virology 176: 505-514, 1990. PubMed: 2161148
Desrosiers RC, Falk LA. Herpesvirus saimiri strain variability. J. Virol. 43: 352-356, 1982. PubMed: 6287011
Biesinger B, et al. Stable growth transformation of human T lymphocytes by herpesvirus saimiri. Proc. Natl. Acad. Sci. USA 89: 3116-3119, 1992. PubMed: 1313581
. Methods in Molecular Genetics, Part A. : K.W. Adolph; 1993.
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