| 產(chǎn)品名稱 |
YEpGAL10-FLP [YEp51-FLP] |
| 商品貨號(hào) |
B213212 |
| Designations |
YEpGAL10-FLP [YEp51-FLP] |
| Species |
Saccharomyces cerevisiae, 2 micron circle |
| Depositors |
JR Broach |
| Applications |
in another host, produces protein recombinase, site-specific |
| Vector |
Construct size (kb): 7.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: YEp51
Intact vector size: 6.800
Type of vector: plasmid
Vector end: SalI
Vector end: HindIII
Cloning sites: HindIII SalI BamHI
Polylinker sites:
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR, LEU2
replicon: pMB1, 2 micron
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiae, 2 micron circle
Gene symbol: FLP
Genomic copy number: unique
Gene name: recombinase, site-specific
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: HaeIII
Insert end: HindIII
Insert size (kb): 0.9
Cross references: Insert lengths(kb): 0.8999999761581421 Gene product: recombinase, site-specific [FLP] Target Gene: recombinase, site-specific |
| Insert Size (kb) |
0.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 2.2 (doublet); HindIII/SalI--7.0, 0.9; SalI--7.8; PstI--6.8, 1.05; XbaI--7.8. Plasmid to eliminate 2 micron circle plasmids and generate cir0 strains by overexpression of FLP. |
| References |
Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781
Volkert FC, Broach JR. Site-specific recombination promotes plasmid amplification in yeast. Cell 46: 541-550, 1986. PubMed: 3524855
|
