For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct information: Total size of construct (kb): 3.9 Marker: ampR Promoter giving the sense strand:T3 Promoter giving the antisense strand: T7 Excise insert: XhoI+NotI
Insert size (kb): 1.0 Gene: lysA, diaminopimelate decarboxylase DNA: genomic DNA Source: Bacillus subtilis Genbank accession: X17013 (Nucleotides 1-1016 of the insesrt correspond to nucleotides 329-1345 of X17013.) Insert end (5’): XhoI linker Insert end (3’): BamHI/polyA/NotI linker
Insert Size (kb)
1.0
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Freeze dried E. coli containing the plasmid
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 329-1345 of Genbank X17013.
An artificial polyA tail was added onto the 3' end of the insert and can be excised using BamHI + NotI. XhoI + BamHI will excise insert without the polyA tail.
The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.16; PstI--0.18; ClaI--0.47; BglI--0.54.
Restriction digests of the clone give the following sizes (kb): NotI--4.0; NotI/XhoI--3.0, 1.1; XhoI--4.0.
