Restriction digests of the clone give the following sizes (kb): HindIII/XbaI--4.0, 3.5; BamHI--4.5, 3.0; AvaI--4.6, 2.0, 0.5, 0.4; EcoRI--3.6, 2.8, 1.2; PstI--2.9, 2.3, 0.9, 0.6, 0.5, 0.4. The insert contains the following restriction sites (nt positions from the 5' end): BamHI--987; XhoI--1246; EcoRI--2816. A full-length cDNA clone extending from nt -33 to +4002 relative to the translation initiation codon. The coding sequence is 3839 nt. When transfected into BRO melanoma cells, the sequence expresses a protein recognized by monoclonal antibodies but does not confer multiple drug resistance. Originally cloned using EcoRI linkers into the polylinker of lambdaZAP. Excised as a HindIII/XbaI fragment (sites from the polylinker) for insertion into pJ3omega. |
