Restriction digests of the clone give the following sizes (kb): SphI--2.7; SacI--2.7; KpnI--2.7; SalI--2.7. Vector with a MCS containing only relatively rare enzyme cutting sites flanked by SfiI sites to facilitate removal of the insert. By reducing the number of sites in the plasmid, more sites in an insert can be used for gene disruption experiments. The order of the major features in this plasmid is: SfiI/SacI/MCS/SfiI/lacZ' - pMB1 ori - ampR. |
