Please note there is an error in the map in the J. Bact. 165: 831-836, 1986 reference. The BclI and EcoRI sites should be reversed.
Shuttle vector for Escherichia coli/Streptococcus faecalis.
pACYC184 and pGB354 joined at AvaI and SphI sites.
Media
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
References
Wirth R, et al. Highly efficient protoplast transformation system for streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector. J. Bacteriol. 165: 831-836, 1986. PubMed: 3005240
