| 產品名稱 |
GC 1657 [GC1657] |
| 商品貨號 |
B216591 |
| Designations |
GC 1657 [GC1657] |
| Species |
Neisseria gonorrhoeae (Zopf) Trevisan |
| Depositors |
Enzo Biochem, Inc., SB Abrams, Enzo Biochem, Inc. |
| Vector |
Construct size (kb): 8.149998664855957 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: M13mp8
Intact vector size: 7.229
Type of vector: phage
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII BalI
BglI PvuI
Polylinker sites: EcoRI SmaI XmaI BamHI SalI AccI HincII PstI HindIII
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
promoter: lac
replicon: M13
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Neisseria gonorrhoeae
Gene name: species-specific DNA
Contains complete coding sequence?: U
Strain: NRL 33152
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 0.85
Cross references: Insert lengths(kb): 0.8500000238418579 Gene product: species-specific DNA |
| Insert Size (kb) |
0.850 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: frozen |
| Comments |
The insert contains the following restrictions sites (in bp from one end): HaeII--220; HindIII--400. It does not contain sites for AvaI, BamHI, BglII, EcoRI, HaeIII, HpaII, SalI or TaqI. Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--7.3, 0.85; EcoRI--8.2; SalI--8.2; BglII--8.2; HindIII--8.2. This probe is highly specific for Neisseria gonorrhoeae and displays no cross-reactivity with N. meningitidis or other members of the Neisseriaceae. |
| References |
Lo A, Yang HL. Nucleotide sequence composition and method for detection of Neisseria gonorrhoeae and method for screening for a nucleotide sequence that is specific for a genetically distinct group. US Patent 4,900,659 dated Feb 13 1990
Donegan JJ, et al. Isolation of a species-specific DNA probe for Neisseria gonorrhoeae using a novel technique particularly suitable for use with closely related species displaying high levels of DNA homology. Mol. Cell. Probes 3: 13-26, 1989. PubMed: 2499769
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
