| 產品名稱 |
AR16 |
| 商品貨號 |
B216955 |
| Designations |
AR16 |
| GenBank Number |
X83543
|
| Species |
Homo sapiens, human |
| Depositors |
MV Schiaffino |
| Applications |
contains sequence apical protein, Xenopus laevis-like |
| Vector |
Construct size (kb): 3.700000047683716 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: APXL
Gene name: apical protein, Xenopus laevis-like
Contains complete coding sequence?: N
Tissue: retina, adult
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.74
Cross references: DNA Seq. Acc.: X83543
Nucleotides 1-741 of the insert correspond to
nucleotides 1-741 of X83543. Insert lengths(kb): 0.7400000095367432 Tissue: retina, adult Gene product: apical protein, Xenopus laevis-like [APXL] Target Gene: apical protein, Xenopus laevis-like |
| Insert Size (kb) |
0.740 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; NotI-- 3.5, 0.3; PvuII--2.5, 0.8, 0.5. The insert contains the following restriction sites (approximate kb from the 5' end): HincII--0.3; NcoI--0.1; NotI--0.2; SalI--0.3; SphI--0.45; StuI--0.4; PvuII--0.15. Contains 90 bp of 5' untranslated sequence and a portion of the coding sequence, overlapping AR17 (ATCC 99510) at the 3' end. The coding region of GenBank X83543 corresponds to nt 91-4941. |
| References |
Schiaffino MV, et al. Cloning of a human homologue of the Xenopus laevis APX gene from the ocular albinism type 1 critical region. Hum. Mol. Genet. 4: 373-382, 1995. PubMed: 7795590
|
