Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
soil
Delaware, United States
Isolation date: October 15, 1992
Product Format
dried
Type Strain
no
Medium
ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Protocol: ATCCNO: 50397 SPEC: Store vial in refrigerator until ready to open it. Open sealed outer glass vial according to instructions provided with the vial. Rehydrate the material by adding 0.5 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048. (Bacterize the medium by inoculating it with a loopful of bacteria from an agar slant at least 24 hours prior to inoculatiion with the flagellate.) Aseptically remove the shredded filter paper pellet with sterile forceps and add a T-25 tissue flask containing 10 ml of bacterized medium. Aseptically remove the remainder of the fluid from the glass vial and add it to the T-25 flask. Screw the cap on tightly and incubate the flask at 25C. Transfer every 21-28 days by vigorously agitating the flask and aseptically transferring 0.1 ml to a fresh flask of bacterized medium.
Subcultivation
Protocol: ATCCNO: 50397 SPEC: Store vial in refrigerator until ready to open it. Open sealed outer glass vial according to instructions provided with the vial. Rehydrate the material by adding 0.5 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC 13048. (Bacterize the medium by inoculating it with a loopful of bacteria from an agar slant at least 24 hours prior to inoculatiion with the flagellate.) Aseptically remove the shredded filter paper pellet with sterile forceps and add a T-25 tissue flask containing 10 ml of bacterized medium. Aseptically remove the remainder of the fluid from the glass vial and add it to the T-25 flask. Screw the cap on tightly and incubate the flask at 25C. Transfer every 21-28 days by vigorously agitating the flask and aseptically transferring 0.1 ml to a fresh flask of bacterized medium.
Name of Depositor
CJ O'Kelly
Chain of Custody
ATCC <<--CJ O'Kelly<<--T.A. Nerad
Year of Origin
October 15, 1992
References
shore of canal running parallel to Brandywine River, Wilmington, DE (39 degrees 45'N, 75 degrees 35'W)
