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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 5.0000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 5.000
Type of vector: plasmid
Cloning sites: BamHI SmaI EcoRI XbaI NcoI SalI XhoI SacI HindIII
Polylinker sites: EcoRI XbaI NcoI SalI XhoI SacI HindIII
Construction: pGEX-2T
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: tac
replicon: pMB1
repressor gene: lacIq

Vector: pGEX-KG (plasmid)

Promoters: Promoter tac

Construction: pGEX-2T

Marker(s):ampR

Construct size (kb): 5.0

Features: marker(s): ampR
promoter: tac
replicon: pMB1
repressor gene: lacIq

expression vector

vector permitting construction of fusion proteins

Restriction digests of the clone give the following sizes (kb): HindIII--5.0; BamHI--5.0; EcoRI--5.0; XbaI--5.0.

Expression vector permitting production of a fusion protein.

The glutathione S-transferase (GST) fusion protein can be purified by glutathione affinity chromatography, and the desired polypeptide released from the fusion product by thrombin.

Constructed from pGEX-2T by inserting an oligonucleotide at the EcoRI site which encodes the glycine "kinker" and additional restriction sites to facilitate cloning in all reading frames.

The order of the major features in this plasmid are: Ptac - GST - thrombin cleavage site - BamHI site - SmaI site - glycine "kinker" - multiple cloning site - ampR - pBR322 ori - lacIq.

Temperature: 37.0°C