-20°C or colder in a manual defrost freezer. Avoid freeze-thawing.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Introduction
Cell Line Description:
The HCC2218 cell line was initiated on April 10, 1996, and took 6 months to establish. HCC2218 was derived from a female whose family members included several members with cancer, four lung cancer patients (two of them, non-smokers), two breast cancer patients (one of them also had lung cancer) and one colon cancer patient. HCC2218 is a poorly differentiated cell line derived from the primary tumor of an invasive ductal carcinoma with metastases in 42/43 lymph nodes. The cells are highly positive for expression of Her2/neu, and are also positive for expression of p53. HCC2218 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 [EGP2] and for cytokeratin 19. The cells are negative for expression of estrogen receptor (ER -), but positive for expression of progesterone receptor (PR +). DNA was isolated from an EBV-transformed lymphoblastoid cell line, HCC2218 BL, from the same patient is available as ATCC CRL-2363D.
Quality Control Specifications
DNA Quality Control Information:
· > 100 kb in size by agarose gel electrophoresis
· No RNA detected in the agarose gel
· A260/A280 ratio 3 1.8
· Tested and verified for PCR amplification and restriction digestion.
