| 產(chǎn)品名稱 |
pMLSV N1/N4-S |
| 商品貨號(hào) |
B223369 |
| Designations |
pMLSV N1/N4-S |
| GenBank Number |
X01057
|
| Species |
Homo sapiens, human |
| Depositors |
Immunex Corporation, S Gillis, Immunex Corporation |
| Applications |
in another host, produces protein interleukin 2 receptor, alpha IL-2R alpha chain, Interleukin-2 receptor, IL-2 receptor |
| Vector |
Construct size (kb): 5.670000076293945 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pMLSV
Intact vector size:
Type of vector: plasmid
Vector end: PstI
Vector end: BglII
Cloning sites: BglII PstI
Polylinker sites: BglII PstI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40 early
replicon: pMB1, SV40 ori
terminator: SV40 polyadenylation
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: IL2RA
Other gene symbols: IL2R
Genomic copy number: unique
Gene name: interleukin 2 receptor, alpha
Contains complete coding sequence?: Y
Chromosome: 10; Localization: 10 p15-p14
Tissue: lymphoma, cutaneous T cell, HUT 102 cell line
Type of DNA: cDNA
Insert 5' end: PstI
Insert 3' end: BamHI
Insert size (kb): 1.79
Cross references: DNA Seq. Acc.: X01057
Nucleotides 1-1790 of the insert correspond to
nucleotides 132-1921 of X01057. Insert lengths(kb): 1.789999961853027 Tissue: lymphoma, cutaneous T cell, HUT 102 cell line Gene product: interleukin 2 receptor, alpha( IL-2R alpha chain, Interleukin-2 receptor, IL-2 receptor) [IL2R] Target Gene: interleukin 2 receptor, alpha |
| Insert Size (kb) |
1.790 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/SstI--2.6, 2.5, 0.5; PstI--3.0, 2.2, 0.4; XbaI--4.7, 0.9; BamHI/PstI--2.3, 2.0, 0.9, 0.42. Expresses a functional IL-2 receptor in COS-7 cells. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.12; PstI--0.43; XbaI--0.87. A SacI/BamHI fragment detects transcripts of 1.4 and 3.4 kb in activated T cells and HUT 102 cells. |
| References |
Cosman D, et al. Cloning, sequence, and expression of human interleukin-2 receptor. Nature 312: 768-771, 1984. PubMed: 6096720
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
