Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Freshwater, Germany, (?)
Product Format
frozen
Storage Conditions
Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures: 2-8°C
Live Cultures: See Protocols section for handling information
Type Strain
no
Comments
Photosynthetic
Medium
ATCC® Medium 5: Sporulation agar
ATCC® Medium 351: Hutner's medium for Euglena
Growth Conditions
Temperature: 20°C to 25°C
Culture System: Axenic
Cryopreservation
Harvest and Preservation
Harvest cells from a culture that is at or near peak density by centrifugation at 400-500 x g for 5 min.
Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium.
While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.
Mix the cell preparation and the 10% DMSO in equal portions. Thus, the final concentration will be 106 - 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and methanol stock solution to the beginning of the freezing process should be no less than 5 min and no greater than 15 min.
Dispense in 0.5 ml aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.
To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial.
Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and add to a centrifuge tube containing 5 mL of ATCC Medium 5 broth (or alternatively, 5 mL ATCC Medium 351 supplemented with 0.1% Na Acetate).
Gently remove most of the supernatant (save in a secondary tube), then resuspend the remaining cells in additional fresh medium to a total volume of 5-6 mL.
Incubate tubes on a 15° horizontal slant with the cap screwed on loosely (loosened one half turn) at 20-25°C under a 14 hour light (~50 µEinsteins/m2/s irradiance)/10 hour dark cycle.
