Restriction digests of the clone give the following sizes (kb): BamHI--4.1; EcoRV--4.1; BstEII/PstI--3.0, 1.1. Expression vector (T7-based) with a kanR marker and a P15A replicon compatible with ColE1-derived plasmids. Particularly useful for co-transformation with ColE1-based ampR T7 expression vectors and the production of two proteins in the same cell. If used in an Escherichia coli strain that expresses T7 polymerase under the control of the lacUV5 promotor (such as BL21(DE3)), addition of IPTG can result in high levels of recombinant protein production. Use of 5'NcoI and 3'BamHI cloning sites is similar to that of other expression systems, which facilitiates transfer of genes into these pMR vectors. Differs from pMR101 (ATCC 87115) by the removal of BglII and Xba sites. |
