| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 9.0, 5.0, 4.3, 1.8, 1.7, 1.7, 0.7; BglII--25.0, 7.0, 3.2, 3.0, 2.4, 1.5, 0.68; PvuI--20.0, 12.0, 8.0; HindIII--21.0, 18.0, 4.3, 0.8; SmaI--20.0, 19.0, 5.6. The insert contains the following sites separated by (kb)(approx): SalI, MboII- 1.5- BamHI- 1.7- BamHI- 1.7- BamHI- 4.3- BamHI- 1.8- BamHI- 0.65- BamHI- 5.0- SmaI- 0.6- BamHI- 0.7- MboII, SalI. Codominant inheritance was observed in 3 informative families (39 children). The 1.6 kb BamHI fragment contains exon 7 and 3' sequences. Exon 7, which has been sequenced, encodes the last 64 amino acids and includes 1.2 kb of 3' non-coding sequence. The BamHI 1.6 doublet detects the TaqI RFLP associated with DRD2. Use standard hybridization conditions with the addition of human placental DNA to block repeat sequences. Washing conditions are standard with a final wash at 0.1 x SSC, 0.1% SDS, 65C. Enzyme(s) not detecting polymorphism: 31 enzymes. |
| References |
Grandy DK, et al. The human dopamine D2 receptor gene is located on chromosome 11 at q22-q23 and identifies a TaqI RFLP. Am. J. Hum. Genet. 45: 778-785, 1989. PubMed: 2573278
Grandy DK, et al. Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. USA 86: 9762-9766, 1989. PubMed: 2532362
Olivier Civelli, personal communication
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