欧美mv日韩mv国产-国产午夜一级片-一区二区在线 | 国-韩国无码一区二区三区免费视频-黄色a免费-欧美黄色大片免费观看-人人综合网-国产成人a在线观看视频免费-国内精品久久久久久久影视-国产老头和老太xxxx视频-高h放荡受浪受bl-国产又爽又黄又不遮挡视频-国产偷国产偷亚洲高清人乐享

Size (kb): 5.9000000953674320

Vector: pSH (plasmid)

Promoters: Promoter T3

Construction: pEN1, BLpSV

Marker(s):ampR

Construct size (kb): 5.900000095367432

Features: marker(s): ampR
promoter: HTLV-1 LTR, T7, T3
replicon: pMB1, SV40
terminator: SV40 polyadenylation
enhancer: HIV-1 LTR

expression vector

shuttle vector

Restriction digests of the clone give the following sizes (kb): EcoRI--5.9; HindIII--5.9; PstI--4.3, 1.6.

Shuttle expression vector utilizing a chimeric promoter derived from the HTLV-I LTR and the HIV-1 LTR. Supports high levels of expression in human and mouse cells.

The order of the major features in the plasmid is: T7 promoter - XhoI - SV40 promoter - HTLV-1 LTR promoter - HIV-1 LTR enhancer - EcoRI/MCS/BglII - SV40 polyadenylation - T3 promoter - pMB1 ori - ampR.

Temperature: 30.0°C