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pDM-17 [pDM17]
pDM-17 [pDM17]
規格:
貨期:
編號:B230782
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pDM-17 [pDM17]
商品貨號 B230782
Designations pDM-17 [pDM17]
Species Homo sapiens, human
Depositors M Barbacid, D Martin-Zanco
Vector
Construct size (kb): 3.799999952316284
Insert
DNA: cDNA
Insert lengths(kb): 1.210000038146973
Tissue: colon carcinoma
Gene product: neurotrophic tyrosine kinase, receptor, type 1( Oncogene TRK, neutrotrophic tyrosine receptor kinase 1 (trk proto-oncogene), proto-oncogene trk) [NTRK1]
Insert Size (kb) 1.210
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.8; EcoRI/BamHI--1.1, 2.7.
pDM10-1 was isolated from a cDNA library from a third cycle NIH/3T3 transformant derived from a human colon carcinoma.
The insert includes nucleotides +854 to +2064. This region includes tyrosine kinase sequences, but not tropomyosin sequences.
This was constructed by inserting the BalI-EcoRI fragment of pDM10-1 into the SmaI-EcoRI sites of the pUC18 polylinker. The insert can be isolated by a BamHI/EcoRI double digest.
References

Martin-Zanca D, et al. A human oncogene formed by the fusion of truncated tropomyosin and protein tyrosine kinase sequences. Nature 319: 743-748, 1986. PubMed: 2869410

Coulier F, et al. Human trk oncogenes activated by point mutation, in-frame deletion, and duplication of the tyrosine kinase domain. Mol. Cell. Biol. 10: 4202-4210, 1990. PubMed: 1695324

Klein R, et al. The trk proto-oncogene encodes a receptor for nerve growth factor. Cell 65: 189-197, 1991. PubMed: 1849459

Mariano Barbacid, personal communication

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