| 產品名稱 |
pBJ5-HD1-F |
| 商品貨號 |
B239252 |
| Designations |
pBJ5-HD1-F |
| GenBank Definition |
Human histone deacetylase HD1 mRNA, complete cds |
| GenBank Number |
U50079
|
| Species |
Homo sapiens, human |
| Depositors |
PG Mark |
| Applications |
In another host, produces protein histone deacetylase 1 |
| Vector |
Name of construct: pBJ5-HD1-F
Excise insert: BamHI
Promoter giving sense strand: SRalpha
Size of construct (kb): 4.8
Markers: ampR
Name of vector: pBJ5
Intact vector size: 3.300
Type of vector: plasmid
Vector end: SacII
Vector end: EcoRI
Cloning sites: NotI SacII SfiI EcoRI
Polylinker sites: NotI SacII SfiI EcoRI BamHI
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter for expression: SRalpha, →
other: 16S splice site, →
MCS: NotI...EcoRI, →
transcription terminator: polyadenylation, →
marker(s): ampR |
| Insert |
Genome: Human
Gene symbol: HDAC1
Gene name: histone deacetylase 1
Contains complete coding sequence?: Y
Chromosome: 1; Localization: 1 p34.1
Tissue: Jurkat T cells
Type of DNA: cDNA
Insert 5' end: NotI, SacII
Insert 3' end: SalI
Insert size (kb): 1.5
Cross references: DNA Seq. Acc.: U50079
Nucleotides ~1-1449 of the insert correspond to nucleotides 111-1559 of U50079.
Insert lengths(kb): 1.5
Tissue: Jurkat T cells
Gene product: histone deacetylase 1 [HDAC1]
Target Gene: histone deacetylase 1 |
| Insert Size (kb) |
1.5 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.3, 1.5; EcoRV--4.8. Produces protein tagged at the C-terminus with the FLAG epitope. |
| References |
Taunton J, et al. A Mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 272: 408-411, 1996. PubMed: 8602529
|
