transformation host for cloning and sequencing of gene encoding NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase on chromosome XI
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATa ser1 ura3-52 trp1 leu2 his4 ade3-30 ade3-65 ade3-144
Preceptrol®
no
Ploidy
haploid
Comments
Does not require adenine in spite of point mutations in ade3 gene
Morphology
After 3 days at 25°C growth is butyrous and light cream colored, cells are globose, ovoidal or elongate, 3.0-8.0 x 5.0-10.0 μm.? Sediment is present after one month.?
Medium
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
Sequenced Data
No DNA sequencing was performed in house on this product.
Name of Depositor
DR Appling
Special Collection
NCRR Contract
Chain of Custody
ATCC <<--DR Appling<<--C.K. Barlowe
References
West MG, et al. Cloning and characterization of the Saccharomyces cerevisiae gene encoding NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase. J. Biol. Chem. 268: 153-160, 1993. PubMed: 8416923
Pasternack LB, et al. C NMR analysis of intercompartmental flow of one-carbon units into choline and purines in Saccharomyces cerevisiae. Biochemistry 33: 74-82, 1994. PubMed: 8286365
