Restriction digests of the clone give the following sizes (kb): BamHI--3.5 (doublet), 2.3; HindIII--9.5; HindIII/EcoRI--6.4, 2.9; HindIII/XhoI--4.1, 3.0, 2.2; PvuII--5.2, 2.3, 1.1, 0.5, 0.4; HindIII/SalI--6.3, 3.3. Contains the entire coding sequence of pro C4A including approximately 60 bp 5' untranslated and approximately 140 bp 3' untranslated sequence plus poly(A). The insert contains an internal BamHI site. The sequence is nearly identical to the C4B gene product. The BglII RFLP is detected using the 400 bp BamHI/KpnI 5' fragment of pAT-A. The polymorphic site maps 2.5 kb upstream from C4A. The 4.2 kb allele segregates with F allele of factor B and the 6.6 kb TaqI fragment in C2. The 2.4 kb BamHI/KpnI fragment can be used to detect C4A gene duplications in different haplotypes when the DNA is digested with TaqI. Enzyme(s) not detecting polymorphism: TaqI, BamHI, KpnI, HindIII, SalI, ClaI. |
