Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
human, Kenya, 1962
Product Format
frozen
Type Strain
no
Comments
Amastigotes.
Growth Conditions
Duration: in vivo, golden hamster
Protocol: ATCCNO: 30499 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. Inject the entire contents of the ampule intraperitoneally into a golden hamster at least 8 weeks old. Serial passage in golden hamster every 30-45 days. The spleen is homogenized in a balanced salt solution (salt solution for ATCC medium 811 is suitable), approximately 5.0 ml solution/spleen. Inoculate 0.3-0.5 ml of spleen homogenate/hamster. Dosage should be a minimum of 2 X 10(6) amastigotes/hamster. The course of infection will vary with the size of the inoculum.
Subcultivation
Protocol: ATCCNO: 30499 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. Upon arrival remove the frozen ampoule from the dry ice and transfer directly to a 35C water bath. Inject the entire contents of the ampule intraperitoneally into a golden hamster at least 8 weeks old. Serial passage in golden hamster every 30-45 days. The spleen is homogenized in a balanced salt solution (salt solution for ATCC medium 811 is suitable), approximately 5.0 ml solution/spleen. Inoculate 0.3-0.5 ml of spleen homogenate/hamster. Dosage should be a minimum of 2 X 10(6) amastigotes/hamster. The course of infection will vary with the size of the inoculum.
Name of Depositor
R Herman
Chain of Custody
ATCC <<--R Herman<<--L. Stauber <<--- H. Hoogstraal
Year of Origin
1962
References
Stauber LA. Characterization of strains of Leishmania donovani. Exp. Parasitol. 18: 1-11, 1966. PubMed: 5909670
