| 產品名稱 |
pSYC1174 [CMCC 2422] |
| 商品貨號 |
B240523 |
| Designations |
pSYC1174 [CMCC 2422] |
| GenBank Number |
I02088
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
Chiron Corp. |
| Applications |
produces protein methionine aminopeptidase |
| Vector |
Construct size (kb): 5.900000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC18
Intact vector size: 2.686
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI
EcoRI
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: HindIII...EcoRI, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: L08752 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Escherichia coli
Gene symbol: map
Gene name: methionine aminopeptidase
Contains complete coding sequence?: Y
Strain: CM89
Type of DNA: genomic
Insert end: Sau3AI
Insert end: Sau3AI
Insert size (kb): 3.2
Cross references: DNA Seq. Acc.: I02088 Insert lengths(kb): 3.200000047683716 Gene product: methionine aminopeptidase [map] Target Gene: methionine aminopeptidase |
| Insert Size (kb) |
3.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--3.4, 2.6; PstI--3.7, 2.2; SmaI--5.9. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,865,974 dated Sep 12 1989
Ben-Bassat A, et al. Processing of the initiation methionine from proteins: properties of the Escherichia coli aminopeptidase and its gene structure. J. Bacteriol. 169: 751-757, 1987. PubMed: 3027045
Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 4,870,017 dated Sep 26 1989
Ben-Bassat A, et al. Bacterial methionine N-terminal peptidase. US Patent 5,013,662 dated May 7 1991
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
