| 產品名稱 |
pSE1111 |
| 商品貨號 |
B241410 |
| Designations |
pSE1111 |
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
SJ Elledge |
| Applications |
in another host, produces protein |
| Vector |
Construct size (kb): 8.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pACT
Intact vector size: 7.650
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BglII BamHI XhoI
Polylinker sites: BglII XhoI BamHI EcoRI BglII
Other unique sites: XbaI HapI ClaI EcoRV
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
replicon: 2 micron
marker(s): LEU2, ->
terminator: ADH
MCS: BglII XhoI ... BglII
other: GAL4 activation domain
promoter for expression: ADH, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharomyces cerevisiaeCandida robusta
Gene symbol: SNF4
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 0.9
Cross references: Insert lengths(kb): 0.8999999761581421 Gene product: [SNF4] |
| Insert Size (kb) |
0.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Related Products |
component of:ATCC 87014 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.2, 0.9; EcoRI--3.7, 3.0, 0.5; BglII--7.0, 0.94. pSE1111 (ATCC 87010) and pSE1112 (ATCC 87011) can be used together as a positive control in 2-hybrid cloning systems. Expresses SNF4 fused to the GAL4 activation domain. |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
Durfee T, et al. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7: 555-569, 1993. PubMed: 8384581
Stephen J Elledge, personal communication
|
