Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Rapid loss in titer when stored above -70°C. Activities with high potential for aerosol production require BSL 3 facilities and practices.
Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated monoclonal antibodies
Recommended Host
McCoy cells (ATCC® CRL-1696™)
Growth Conditions
Temperature: 35.5°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 95-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 2100 x g for 1 hour at 20°C. End adsorption by adding growth medium. Incubation: 3 days
Name of Depositor
L Hanna, E Jawetz, Centers for Disease Control and Prevention
Source
Eye of nine-day-old baby, California, 1960
References
Hanna L, et al. Isolation of viruses from inclusion conjunctivitis in the newborn. Am. J. Ophthamol. 53: 774-780, 1962. PubMed: 13904377
