| 產品名稱 |
pDP252 |
| 商品貨號 |
B241817 |
| Designations |
pDP252 |
| Species |
Hepatitis B virus, Hepatitis B virus |
| Depositors |
Health Research, Inc., D Panicali, Health Research, Inc. |
| Applications |
This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims. |
| Vector |
Construct size (kb): 5.440000057220459 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: HindIII
Vector end: HindIII
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: hepatitis B virus
Gene symbol: sAg
Gene name: surface antigen
Contains complete coding sequence?: U
Type of DNA: unknown
Insert end: HhaI
Insert end: HhaI
Insert size (kb): 1.08
Cross references: Insert lengths(kb): 1.080000042915344 Gene product: surface antigen [sAg] |
| Insert Size (kb) |
1.080 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The insert contains a BamHI site. Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 1.15; EcoRI--5.7. The insert contains a BamHI site. This material is cited in a U.S. and/or other Patent and may not be used to infringe the patent claims. Constructed by cloning a 1.084 kb HhaI fragment of HBV into pBR322 This clone was constructed by isolating a 1.084 kb HhaI fragment from pTHBV containing the coding sequence for the surface antigen without pre-surface antigen. This was blunt-ended with T4 DNA polymerase, linkered with HindIII, and inserted into pBR322. |
| Classification |
Hepadnaviridae, Orthohepadnavirus |
| References |
Paoletti E, Panicali D. Modified vaccinia virus. US Patent 4,603,112 dated Jul 29 1986
Paoletti E, Panicali D. Method for immunizing animals with synthetically modified vaccinia virus. US Patent 4,722,848 dated Feb 2 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
